TransScript^® II One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover is combined with TransScript^® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.

• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.

• cDNA up to 15 kb.

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

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