Products

RNA Library Prep
  • KP801-01.jpg
KP801-01.jpg

TransNGS® RNA-Seq Library Prep Kit for MGI®


Product Details

The kit is designed for the MGI high-throughput sequencing platform to prepare strand-specific or non-strand-specific transcriptome libraries. The applicable sample is 0.1 ng-100 ng of processed RNA (mRNA captured by mRNA magnetic beads or RNA obtained after rRNA removal) to prepare a high-quality sequencing library with high yield and complete information. The kit completes the second-strand cDNA synthesis, end repair and A-tailing in one step, without the purification steps, which greatly simplifies the operation process and shortens the library preparation time, and can complete the preparation of high-quality sequencing libraries within 3 hours .


Storage

at 2-8°C for one year



Shipping

Dry ice (-70℃)


Product Contents


ComponentKP801-01 (12 rxns)KP801-02 (96 rxns)
4×RNA Fragmentation Buffer II54 μl432 μl
Library First-Strand Buffer II72 μl576 μl
Library First-Strand Enzyme Mix36 μl288 μl
Library SEA Buffer II(+dUTP)300 μl4×600μl
Library SEA Buffer II300 μl4×600μl
Library SEA Enzyme Mix120 μl960 μl
Adapter Dilution Buffer600 μl5 ml
Adapter Ligation Buffer II360 μl4×720 μl
Adapter Ligation Enzyme60 μl480 μl
TransNGS® Library Amplification SuperMix (2×)300 μl4×600 μl
TransNGS® Universal Primer for MGI60 μl480 μl
Uracil-DNA Glycosylase12 μl96 μl
Nuclease-free Water2×1 ml3×5 ml


CopyRight 2022 TransGen Biotech Co., Ltd. All Rights Reserved          京ICP备07502849号-1       京公网安备 11010802016705号
Message