The kit is designed for the MGI high-throughput sequencing platform to prepare strand-specific or  non-strand-specific transcriptome libraries. The applicable sample is 0.1 ng-100 ng of processed RNA (mRNA  captured by mRNA magnetic beads or RNA obtained after rRNA removal) to prepare a high-quality sequencing  library with high yield and complete information. The kit completes the second-strand cDNA synthesis, end  repair and A-tailing in one step, without the purification steps, which greatly simplifies the operation process  and shortens the library preparation time, and can complete the preparation of high-quality sequencing libraries  within 3 hours .

Features 

 • No actinomycin D, no need to avoid light, non-toxic operation, higher strand-specific ratio. 

 • High library conversion rate, high library yield, and high data quality. 

Application 

 • Whole transcriptome sequencing 

 • Gene expression analysis 

 • Single nucleotide variation analysis 

 • Variable splicing testing 

 • Fusion genetic testing 

 • Analysis of non-coding RNA and RNA precursor

Storage

at -20℃ for one year

Shipping

Dry ice (-70℃)