The product contains PerfectStart^® Fast Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer, PCR stabilizer and tracer dye. Three monoclonal antibodies bind to the FastTaq DNA Polymerase engineered by directed evolution with high affinity, which are prepared by optimized process, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature. The qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, Universal Passive Reference Dye (optional) and nuclease-free water. This product uses the principle of mixing yellow and blue to generate green color to track the pipetting process. The qPCR SuperMix contains a yellow dye, and 10×Sample Buffer contains a blue dye. Adding template diluted in Sample Buffer with a brighter color (bright blue) to the qPCR SuperMix (light yellow) will show an obvious color change from yellow to green with easy naked-eye visualization which is more effective in avoiding pipetting errors.

• PerfectStart^® Fast Taq DNA Polymerase: fast and efficient; hot-start and blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.

• Dual-cation buffer enhances specificity and reduces primer-dimer formation.

• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to slight variations in reaction volume or differences in well position.

• Contain tracer dye to avoid pipetting errors.