PerfectStart® IV Fast Probe qPCR SuperMix UDG is supplied at a 2× concentration, and is a pre-mix reagent for the amplification and detection of DNA in probe-based qPCR. Simply add primers, probe and template to perform singleplex, duplex and multiplex PCR reactions in one reaction well. This product contains newly upgraded PerfectStart® IV Taq Hot-Start DNA Polymerase (Genetically modified next generation Taq DNA polymerase, using antibody blocking method to effectively block the activity of DNA polymerase to prevent non-specific amplification at low temperature), combined with optimal reaction buffer, which significantly improves template affinity, qPCR amplification performance and the sensitivity of low-concentration template detection. It has strong resistance to impurity samples, which is suitable for a variety of detection scene. This product can support fast amplification, enabling 45 cycles of PCR amplification to be completed in 35 minutes. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and ensure the accuracy of results.

Features 

• Hot start Taq DNA Polymerase blocking by antibodies, enabling high specificity, high sensitivity, high amplification efficiency, and a wide range of applicable species. 

• High-efficiency antibody blocking supports full premixing of primers and probes, high anti-inhibition ability. This reagent can be used for direct amplication from blood, tissue homogenate, swab and other samples. 

• Supports fast amplification, enabling 45 cycles of PCR amplification to be completed in 35 minutes. 

• The dUTP/UDG anti-contamination system is used to effectively prevent carry-over contamination of PCR products, ensuring the accuracy of results. 

• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors

Applications 

Detection of DNA of animal, plant and microorganism by probe fluorescence quantitative PCR

Storage

at -20˚C for two years 

Shipping

Dry ice (-70℃)