This product contains high-concentration Bst II DNA polymerase and premixed LAMP reaction buffer, suitable for LAMP reaction with DNA as template.

Bst II DNA Polymerase is a recombinant Bacillus stearothermophilus DNA polymerase, which is obtained by purification and isolation after expression in E. coli. The enzyme has 5'→3' DNA polymerase activity and lacks 5'→3' exonuclease activity.

5×LAMP Reaction Buffer is an optimized LAMP reaction solution, which already contains MgSO4, dNTPs and other components required for the reaction, and no additional addition is required.

5× LAMP Reaction Buffer does not contain glycero, the glycerol concentration in the stock solution of Bst II DNA Polymerase (160 U) is 50%. The reaction system can be used for lyophilization.

TS LAMP Green is a DNA-binding dye for fluorescence quantitative amplification. It has a similar spectrum to SYBR Green I and is compatible with all well-known brands of qPCR instruments. Replacing SYBR Green I with TS LAMP Green does not require any changes to current procedures and equipment.

Features 

• Isothermal Amplification (LAMP) capability 

• Fast polymerization 

• Strong strand-displacement capability

Application

• DNA isothermal amplification

• DNA sequencing with GC-rich regions

• Applicable for experiments requiring mesophilic strand-displacement

Storage

at -20℃ for two years

Shipping

Dry ice (-70℃)