ProteinFind® Anti-CD3ε Mouse Monoclonal Antibody
Catalog Number： HI101-01
Price：Please inquire first
Product Details
The mammalian CD3 molecule, which is expressed on the surface of mature T lymphocytes, is an important surface marker of T cell population and mainly consists of δ, ε, γ and ζ chains. CD3 molecules are non-covalent bonded to T-cell receptor (TCR) to form a TCR-CD3 complex, and when antigen presenting cells (APCs) activate TCR, TCR-mediated signals are transmitted to the cell interior via CD3δ, CD3ε, CD3γ, or CD3ζ. Cytoplasmic regions of all CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs), which are phosphorylated by the Src family proteins tyrosine kinases LCK and FYN once bound to TCR, thus activating downstream signaling pathways[1]. In addition to its signal transduction role in T cell activation, CD3ε also plays a critical role in the proper development of T cells. The formation of two heterodimers, CD3δ/CD3ε and CD3γ/CD3ε, initiates the assembly of TCR-CD3 complex, and special sequences in the cytoplasmic region of CD3ε are involved in the internalization of TCR-CD3 complex, down-regulating the expression level of TCR cell surface [2, 3].
Storage
PBS (pH7.4), 0.05% Sodium Azide, 1% BSA, 40% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.
Shipping
Dry ice (-70℃)
[1] Barber EK, Dasgupta JD, Schlossman SF, et al. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex [J]. Proc Natl Acad Sci U S A. 1989, 86(9): 3277-81.
[2] Borroto A, Lama J, Niedergang F, et al. The CD3 epsilon subunit of the TCR contains endocytosis signals [J]. J Immunol. 1999, 163(1): 25-31.
[3] Martin-Blanco N, Jiménez Teja D, Bretones G, et al. CD3ε recruits Numb to promote TCR degradation [J]. International Immunology. 2016, 28(3): 127-37.
