TransNGS® Tn5 DNA Library Prep Kit for Illumina® (for 50 ng DNA)
Catalog Number： KP101-11
Price：Please inquire first
Product Details
TransNGS® Tn5 DNA Library Prep Kit for Illumina® is a reagent kit designed for Illumina high-throughput sequencing platform, suitable for preparing DNA library from 50 ng initial sample. The resulting library can be used for single-end or paired-end sequencing. This kit employs an in vitro transposition technique, which can simultaneously complete DNA fragmentation and adapter ligation within 5 minutes. In comparison to conventional library preparation method that requires DNA fragmentation, end
repair, and adapter ligation, the in vitro transposition technique significantly shortens library construction time and simplifies the operation.
• Time saving and simple operation
• Require small amount of DNA
Prepare short fragment DNA library for Illumina high-throughput sequencing platform from purified dsDNA
at -18℃ or below for one year
Dry Ice (-70℃)
Component | KP101-11 (12 rxns) | KP101-03 (96 rxns) |
| Tn5-50 Enzyme Mix | 24 μl | 192 μl |
| 5×Insertion Buffer | 75 μl | 600 μl |
| 2×Tn5 Digestion Mix | 375 μl | 4×750 μl |
| TransNGS® Tn5 Library Amplification SuperMix (2×) | 300 μl | 4×600 μl |
| Library Elution Buffer | 1.2 ml | 2×5 ml |
| Nuclease-free Water | 1 ml | 5 ml |
1 Pei H, Teng W, Gao L, et al. Low-affinity SPL binding sites contribute to subgenome expression divergence in allohexaploid wheat[J]. Science China Life Sciences, 2023.(IF 10.37)
