TransNGS® Tn5 DNA Library Prep Kit for Illumina® (for 50 ng DNA)
Catalog Number： KP101-11
Price：Please inquire first
Product Details
TransNGS® Tn5 DNA Library Prep Kit for Illumina® is a reagent kit designed for Illumina high-throughput sequencing platform, suitable for preparing DNA library from 1ng initial sample (such as acquiring small genome, plasmid and PCR amplification product larger than 300 bp). The resulting library can be used for single-end or paired-end sequencing. This kit employs an in vitro transposition technique, which can simultaneously complete DNA fragmentation and adapter ligation within 5 minutes. In comparison to conventional library preparation method that requires DNA fragmentation, end repair, and adapter ligation, the in vitro transposition technique significantly shortens library construction time and simplifies the operation.
• Time saving and simple operation
• Require small amount of DNA
Prepare short fragment DNA library for Illumina high-throughput sequencing platform from purified dsDNA
At -20℃ for one year
Dry Ice (-70℃)
Component | KP101-11 (12 rxns) | KP101-03 (96 rxns) |
| Tn5-50 Enzyme Mix | 24 μl | 192 μl |
| 5×Insertion Buffer | 75 μl | 600 μl |
| 2×Tn5 Digestion Mix | 375 μl | 4×750 μl |
| TransNGS® Tn5 Library Amplification SuperMix (2×) | 300 μl | 4×600 μl |
| Library Elution Buffer | 1.2 ml | 2×5 ml |
| Nuclease-free Water | 1 ml | 5 ml |
1 Pei H, Teng W, Gao L, et al. Low-affinity SPL binding sites contribute to subgenome expression divergence in allohexaploid wheat[J]. Science China Life Sciences, 2023.(IF 10.37)
