Products

DNA Library Prep
  • KP221.jpg
KP221.jpg

TransNGS® DNA Library Prep Kit for MGI®


Product Details

The kit is designed to efficiently and quickly prepare DNA library from 1 ng to 1 μg fragmented double-stranded DNA for the MGI high-throughput sequencing platform. The samples can be fragmented dsDNA such as ultrasonic fragmented genomic DNA, enzyme digestion products, nucleic acid amplification products, chromatin immunoprecipitation DNA (ChIP DNA), formalin-fixed paraffin-embedded DNA (FFPE DNA), and can be combined with exon or other target capture reagents for exon sequencing or target capture sequencing.


Application 

• Whole genome sequencing. 

• Target gene sequencing. 

• Exon sequencing / other targeted capture sequencing. 

• Metagenomic sequencing.

• ChIP sequencing.


Storage

at -20°C for one year



Shipping

Dry ice (-70℃)


Product Contents


ComponentKP221-01 (12 rxns)KP221-02 (96 rxns)
End-repair and A-tailing Reaction Mix120 μl960 μl
End-repair and A-tailing Enzyme Mix II60 μl480 μl
End-repair and A-tailing Enhancer36 μl288 μl
Adapter Dilution Buffer600 μl5 ml
Adapter-ligation Buffer II360 μl4×720 μl
Adapter-ligation Enzyme60 μl480 μl
TransNGS® Library Amplification SuperMix (2×)300 μl4×600 μl
TransNGS® Universal Primer for MGI®60 μl480 μl
Library Elution Buffer2 ml4×4 ml
Nuclease-free Water1 ml5 ml


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