pEASY®-Blunt Zero Cloning Kit
Catalog Number： CB501-01
Price：Please inquire first
Product Details
pEASY®-Blunt Zero Cloning Vector contains a suicide gene. When the insert is successfully connected to the vector, the expression of the suicide gene will be disrupted, and the host cell can grow normally, otherwise the host cell will die, thus realizing the "Zero" background. It is suitable for blunt-end cloning.
Features
• Fast: 5 minutes.
• Simple: Just add the fragment.
• High cloning efficiency: Positive rate is close to 100%.
• The background is clean.
• No blue/white selection needed.
• Suitable for cloning both short and long fragments.
• Kanamycin and Ampicillin resistance genes for selection.
• M13 forward primer and M13 reverse primer for sequencing.
• T3 promoter and T7 promoter for in vitro transcription.
• Trans1-T1 competent cells have high transformation efficiency and rapid growth rate, ensuring the number of clones and saving
screening time.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70℃ or below for six months; others at-18℃ or below for nine months
Shipping
Dry ice (-70℃)
| Component | CB501-01 (20 rxns) | CB501-02 (60 rxns) |
| pEASY ®-Blunt Zero Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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