The TransDetect^® Dual-Luc Pro Luciferase Reporter Assay Kit is a highly sensitive, homogeneous dual-luciferase reporter detection kit with no irritating smell and exceptionally stable luminescent signals. This kit contains high-purity firefly luciferin and coelenterazine, enabling sequential detection of firefly luciferase and Renilla luciferase in the same sample: first, the firefly luciferase signal is measured using firefly luciferin as substrate, then the signal is quenched, followed by detection of Renilla luciferase luminescence using coelenterazine as substrate—allowing for dual-luciferase reporter assays. Compared to flash-type TransDetect^® Double-Luciferase Reporter Assay Kit(FR201), this product is easier to use, enables add-read assay protocol without the need for removal of the medium, and provides more stable glow-type signal.

• Stable luminescent signal: Half-life is about 2 h (20-25℃), suitable for high-throughput detection. 

• Accurate result: The Renilla luciferase gene is used as an internal control to correct for variations in cell number between wells and transfection efficiency. 

• Easy to use: Without the need for removal of the medium, and the detection reagent can be added directly. 

• No irritating smell.

The unopened kit can be stored at -18℃ or below for one year. The reconstituted Dual-Luc Pro Luciferase Reaction Reagent can be stored at -70℃ or below for one year, or at -18℃ or below for no more than one month, and avoid repeated freezing and thawing. Dual-Luc Pro Luciferase Reaction Reagent II should be freshly prepared before use.

Dry ice (-70℃)