TransNGS® Single Cell Full Length cDNA Synthesis&Amplification Kit is a reagent kit capable of obtaining amplified full-length cDNA from single cell. It is suitable for amplifying and constructing cDNA libraries from 1-500 cells or 10 pg-10 ng total RNA. The kit uses Single Cell Oligo (dT) as the reverse transcription primer and employs a reverse transcriptase with high synthesis efficiency and template-switching activity to add a special sequence to the 3' end of the cDNA, thereby obtaining full-length cDNA products. The kit is compatible with various cell types and tissue types, suitable for cell materials with varying RNA content. Typically, a single-cell library can yield 10-60 ng of amplified cDNA products.
Storage
Single cell TSO should be stored at -70℃ for one year. The other components should be stored at -20℃ for one year.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
