RT-PCR
  • AH401-01.JPG
AH401-01.JPG

TransScript® II Two-Step RT-PCR SuperMix


Product Details

TransScript® II Two-Step RT-PCR SuperMix performs first-strand cDNA synthesis and PCR in two steps. 5×TransScript® II All-in-One SuperMix for PCR is used for reverse transcription and 2×TransTaq® HiFi PCR SuperMix II is used for PCR.

Amplification of fragment up to 15 kb.

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH401-01
TransScript® II All-in-One SuperMix for PCR200 μl
TransTaq® HiFi PCR SuperMix II2×1 ml
RNase-free Water1 ml


Citations

Sun D , Zhang Y , Wang C , et al. Sox9-related signaling controls zebrafish juvenile ovary–testis transformation[J]. Cell Death and Disease, 2013, 4(11): e930.

Hu W , Xiang Q , Wen Z , et al. Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia[J]. Molecular Medicine Reports, 2014, 9(6):2573-2581.

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