RNA Library Prep
  • KD101-01.JPG

TransNGS® rRNA Depletion Kit (Human/Mouse/Rat)

Product Details

TransNGS® rRNA Depletion Kit (Human/Mouse/Rat) depletes ribosomal RNA from human/mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.

• The kit can remove up to 99% ribosomal RNA from human/mouse/rat total RNA.
• Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of non-ribosomal RNA.

at -20℃ for one year.

Dry ice (-70℃). 

Product Contents

ComponentKD101-01 (6 rxns)KD101-02 (24 rxns)KD101-03 (96 rxns)
rRNA Probe (H/M/R)12 μl48 μl192 μl
E.coli RNase H6 μl24 μl96 μl
DNase I30 μl120 μl480 μl
5×Hybridization Buffer18 μl72 μl288 μl
10×RNase H Reaction Buffer12 μl48 μl192 μl
10×DNase I Reaction Buffer30 μl120 μl480 μl
RNase-free Water500 μl2×1 ml8×1 ml
Control qPCR Primer SetsRibosomal RNA Primer Mix12 μl48 μl192 μl
Non-ribosomal RNA Primer Mix12 μl48 μl192 μl

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