Restriction Enzymes

FlyCut® BsgI


Product Details

FlyCut® BsgI is expressed and purified from E.coli that carries the recombinant BsgI gene. The molecular weight is 122 kDa, with the recognition site at GTGCAG(N)14∨(N)2∧ . The reaction is conducted for 5-15 minutes at 37℃ , and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GTGCAG(N)16 ...3’
3’...CACGTC(N)14 ...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJB301-01JB301-02
FlyCut® BsgI50 units2×50 units
10×FlyCut® Buffer1 ml1 ml
10×DNA Loading Buffer1 ml1 ml


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