One-step (Complete all reactions from reverse transcription to qPCR in a single step within the same reaction system)

Basic Model

TransScript^® Green

AQ211

Dye-based

• High-copy and low-copy gene detection
• Detection of RNA viruses and low-abundance RNA

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TransScript^® Probe

AQ221

Probe-based

×

Star Model

TransScript^®Ⅱ Green

AQ311

Dye-based

• High-copy and low-copy gene detection
• Detection of RNA viruses and low-abundance RNA

×

TransScript^®Ⅱ Probe

AQ321

Probe-based

×

TransScript^® II Multiplex Probe

AQ322

Multiplex probe-based

• 1- to 4-plex qRT-PCR detection
• High-copy and low-copy gene detection
• RNA templates with high GC content or complex secondary structures
• Detection of RNA viruses and low-abundance RNA

√

Two-step

Star Model

PerfectStart^® Uni

AUQ

Dye-based

• 5-minute reverse transcription with simultaneous genomic DNA removal
• qPCR amplification uses PerfectStart® core enzyme, offering high amplification efficiency, good specificity, and accurate data

×

miRNA

TransScript^® Green miRNA

AQ202

Dye-based

• Optimized ratio of tailing enzyme and reverse transcriptase ensures reverse transcription efficiency
• Poly(A) tailing and cDNA synthesis are completed in one step within the same reaction system
• qPCR amplification uses PerfectStart® core enzyme, offering high amplification efficiency, good specificity, and accurate data

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