TransScript® II Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® II All-in-One SuperMix for qPCR together with gDNA remover allows simultaneous first-strand cDNA synthesis and gDNA removal at 42℃ -55℃ . 5×TransScript® II All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.
Highlights
• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.
• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
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PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
Instruments
SARS-CoV-2 Detection
Residual DNA Detection
Animal Disease
