TransScript® II Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® II All-in-One SuperMix for qPCR together with gDNA remover allows simultaneous first-strand cDNA synthesis and gDNA removal at 42℃ -55℃ . 5×TransScript® II All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.
Highlights
• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.
• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
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PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Stem Cell Related
Antibodies
Instruments
SARS-CoV-2 Detection
