qPCR and qRT-PCR SuperMixes
  • AQ301-01.JPG
AQ301-01.JPG

TransScript® II Green Two-Step qRT-PCR SuperMix


Product Details

TransScript® II Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® II All-in-One SuperMix for qPCR together with gDNA remover allows simultaneous first-strand cDNA synthesis and gDNA removal at 42℃ -55℃ . 5×TransScript® II All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.


Highlights

• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.

• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.

• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).


Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ301-01
TransScript® All-in-One SuperMix for qPCR200 μl
TransScript® All-in-One No-RT Control SuperMix for qPCR20 μl
gDNA Remover50 μl
PerfectStartTM Green qPCR SuperMix3×1 ml
Passive Reference Dye (50×)120 μl
RNase-free Water1 ml


Citations

Chen X , Fan Z , Mcgee W , et al. TDP-43 regulates cancer-associated microRNAs[J]. Protein & Cell, 2018, 9(10):848-866.

Zhao L , Ding Q , Zeng J , et al. An Improved CTAB–Ammonium Acetate Method for Total RNA Isolation from Cotton[J]. Phytochem Anal, 2012, 23(6):647-50.

Li S , Jia X , Wen J . Improved 2-methyl-1-propanol production in an engineeredBacillus subtilisby constructing inducible pathways[J]. Biotechnology Letters, 2012, 34(12):2253-2258.

Jiang Y , Wang L . Role of histone deacetylase 3 in ankylosing spondylitis via negative feedback loop with microRNA-130a and enhancement of tumor necrosis factor-1α expression in peripheral blood mononuclear cells[J]. Molecular Medicine Reports, 2015, 13(1):35-40.

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