qPCR and qRT-PCR SuperMixes
  • AQ201-01.JPG
AQ201-01.JPG

TransScript® Green Two-Step qRT-PCR SuperMix


Product Details

TransScript® Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® All-in-One SuperMix for qPCR together with gDNA Remover allows simultaneous first-strand cDNA synthesis and gDNA removal. 5×TransScript® All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.


Highlights

• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.
• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
•Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions). 


Applications
Multiple copy and low copy gene detection.

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAQ201-01
TransScript® All-in-One SuperMix for qPCR200 μl
TransScript® All-in-One No-RT Control SuperMix for qPCR20 μl
gDNA Remover50 μl
PerfectStartTM Green qPCR SuperMix3×1 ml
Passive Reference Dye (50×)120 μl
RNase-free Water1 ml


Citations

Hongtai Z , Zhaogang S , Wenjing W , et al. Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq[J]. PLoS ONE, 2014, 9(2):e88909.

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