Plasmid Extraction Product Selection Guide

Category

Cat.No.

Sample Volume

Maximum Yield

Downstream Application

Features

MiniPrep

EM101

E. coli cultured in ≤20 mL of LB medium

40 μg

Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc.

Visualized operation and high DNA purity

EM111

Suitable for experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

Fast: Extraction completed within 1 hour.
Simple: Endotoxin removal via column.
• High DNA purity with no endotoxin.
• Visualized operation.

EM112

5 ml to 60 ml of E. coli culture

700 μg

Suitable for  experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• Visualized operation.
• Simple and time-saving operation: Endotoxin removal by ER requires no ice-bath, water-bath, or centrifugation steps.

Endotoxin-free: The purified plasmid has an endotoxin level of <0.1 EU/μg.

• High purity: Enables preparation of high-purity plasmid DNA with no RNA residue.

MaxiPrep

EM122

E. coli cultured in ≤500 mL of LB medium

4 mg

Suitable for experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• Visualized operation.
• Recommended for extraction from 100–200 mL of bacterial culture.
Fast: Extraction completed within 1 hour.
Simple: Endotoxin removal by push-filtration.

EM123

E. coli cultured in ≤500 mL of LB medium

Suitable for experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• Visualized operation.
• Recommended for extraction from 100–200 mL of bacterial culture.
Fast: Extraction completed within 1 hour.
High purity: Produces high-purity, endotoxin-free (<0.1 EU/μg, as low as 0.001 EU/μg) transfection-grade plasmid DNA.

EM152

E. coli cultured in ≤500 mL of LB medium

5 mg

Suitable for experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• Visualized operation.
All-in-one tube: Lysis and neutralization of 100–500 mL of bacterial cells can be completed within a single 50 mL centrifuge tube, eliminating the need for large-volume consumables.
Fast: Recommended for extraction from 200–500 mL of bacterial culture. The small-volume format combined with a large spin column significantly reduces centrifugation steps and saves operation time.

EM153

E. coli cultured in ≤500 mL of LB medium

Suitable for experiments  including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• Visualized operation.

Endotoxin-free: Endotoxin levels <0.1 EU/μg, as low as 0.001 EU/μg.

• All-in-one tube: Lysis and neutralization of 100–500 mL of bacterial cells can be completed within a single 50 mL centrifuge tube, eliminating the need for large-volume consumables.

Fast: Recommended for extraction from 200–500 mL of bacterial culture. The small-volume format combined with a large spin column significantly reduces centrifugation steps and saves operation time.

Automation

EM131-96

E. coli cultured in ≤3 mL of LB medium or 2×YT/TB medium

/

Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc.

• Simple operation, no centrifugation required, and fast extraction.
• Stable extraction yield and high purity.
Compatible with vortex mixers and 96-channel nucleic acid extractors.

EM141-24-11-AS

≤45 mL of E. coli culture or ≤0.3 g of cell pellet sample

/

Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc.

• Simple operation, no centrifugation required, and fast extraction.
• Stable extraction yield and high purity.
Compatible for combined use with vortex mixers and 24-channel automated nucleic acid extractors.

Vacuum Manifold

EV101

A vacuum filtration auxiliary device for maxi plasmid extraction that eliminates multiple centrifugation steps and tedious operations such as waste liquid removal and cap handling.

• Contamination and backflow prevention: Unique structural design prevents backflow and ensures no contamination.
• Flexible scalability: Parallel connection enables flexible compatibility with various sample throughputs.
• Easy maintenance: Constructed from durable, wear-resistant, and corrosion-resistant materials, and is easy to clean.

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