Plasmid Extraction Product Selection Guide
Category | Cat.No. | Sample Volume | Maximum Yield | Downstream Application | Features |
MiniPrep | E. coli cultured in ≤20 mL of LB medium | 40 μg | Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc. | Visualized operation and high DNA purity | |
Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Fast: Extraction completed within 1 hour. | ||||
5 ml to 60 ml of E. coli culture | 700 μg | Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Visualized operation. • Endotoxin-free: The purified plasmid has an endotoxin level of <0.1 EU/μg. • High purity: Enables preparation of high-purity plasmid DNA with no RNA residue. | ||
MaxiPrep | E. coli cultured in ≤500 mL of LB medium | 4 mg | Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Visualized operation. | |
E. coli cultured in ≤500 mL of LB medium | Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Visualized operation. | |||
E. coli cultured in ≤500 mL of LB medium | 5 mg | Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Visualized operation. | ||
E. coli cultured in ≤500 mL of LB medium | Suitable for experiments including restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc. | • Visualized operation. • Endotoxin-free: Endotoxin levels <0.1 EU/μg, as low as 0.001 EU/μg. • All-in-one tube: Lysis and neutralization of 100–500 mL of bacterial cells can be completed within a single 50 mL centrifuge tube, eliminating the need for large-volume consumables. • Fast: Recommended for extraction from 200–500 mL of bacterial culture. The small-volume format combined with a large spin column significantly reduces centrifugation steps and saves operation time. | |||
Automation | EM131-96 | E. coli cultured in ≤3 mL of LB medium or 2×YT/TB medium | / | Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc. | • Simple operation, no centrifugation required, and fast extraction. |
EM141-24-11-AS | ≤45 mL of E. coli culture or ≤0.3 g of cell pellet sample | / | Suitable for experiments including restriction enzyme digestion, ligation, transformation, and sequencing, etc. | • Simple operation, no centrifugation required, and fast extraction. | |
Vacuum Manifold | EV101 | A vacuum filtration auxiliary device for maxi plasmid extraction that eliminates multiple centrifugation steps and tedious operations such as waste liquid removal and cap handling. | • Contamination and backflow prevention: Unique structural design prevents backflow and ensures no contamination. | ||