This product is universal column-based plasmid maxiprep kit. It uses a modified lysis system to lyse E.coli cells to efficiently release plasmid DNA, followed by the binding of the DNA onto the silica membrane spin column. Lysis and neutralization of 100-500 ml E.coli liquid cultured in LB medium can be completed in a single centrifuge tube. The volume of the supernatant is less than 50 ml. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the operation. The new endotoxin removal solution can reduce endotoxin levels to as low as 0.001 EU/μg. The purified DNA is suitable for restriction enzyme digestion, ligation, transformation, sequencing, and transfection, etc.

• 1-tube type: Lysis and neutralization of 100-500 ml bacterial solution can be completed in a single 50 ml centrifuge tube, without the need to prepare large-volume consumables. 

• Visualization: Solution LB III (blue) indicates whether lysis and neutralization are complete through the change of color, ensuring the quality of plasmid extraction. 

• Fast: The universal system is compatible with extraction of 100-500 ml bacterial solution. Small volume system combined with large volume spin column greatly reduces the number of centrifugation steps and saves operation time. 

• Endotoxin-free: Purified plasmid with endotoxin levels <0.1 EU/μg (as low as 0.001 EU/μg).

• High yield: Up to 5 mg nucleic acid load in the purification column.

at 15℃-30℃ for 18 months.