PCR Enzyme & SuperMix Series Product Selection Guide

Application Scenarios

Series

Product Form

Cat.No.

Fidelity

(VS EasyTaq®)

Hot-start

Amplification

 Speed

Amplicon Lengt

 (gDNA)

Product End

 Structure

Standard PCR

Rapid Amplification

TransFast®

Enzyme

AP101

-

<2 kb 1 sec/kb;

≥2kb 10 sec/kb

≤4 kb

3'-dA

SuperMix

AS102

≤6 kb

Standard PCR Amplification

EasyTaq®

Enzyme

AP111

-

1-2 kb/min

≤4 kb

3'-dA

SuperMix

AS111

Short Fragment Amplification, PAGE Detection

EasyTaq® for PAGE

Enzyme

AP112

-

1-2 kb/min

≤3 kb

3'-dA

SuperMix

AS112

High-Fidelity PCR

Complex Template Amplification

TransTaq® -T

Enzyme

AP122

18×

1-2 kb/min

≤8 kb

3'-dA

SuperMix

AS122

GC/AT-rich template amplification, complex templates, long-fragment PCR

TransTaq® HiFi

Enzyme

AP131

18×

1-2 kb/min

≤15 kb

3'-dA

SuperMix

AS131

qPCR, Multiplex PCR, SNP
GC/AT-rich template amplification
Complex template, long-fragment amplification

TransStart® Taq

Enzyme

AP141

18×

1-2 kb/min

≤15 kb

3'-dA

qPCR, Multiplex PCR, SNP
GC/AT-rich template amplification
Complex template, long-fragment amplification

TransStart® TopTaq

Enzyme

AP151

18×

1-2 kb/min

≤15 kb

3'-dA

Low-copy template, complex template
qPCR, Multiplex PCR, SNP, GC/AT-rich template amplification

PerfectStart® Taq

Enzyme

AP401

18×

1-2 kb/min

≤1 kb

3'-dA

Gene Site-Directed Mutagenesis

TransStart® KD Plus

Enzyme

AP301

108×

1 kb/min

≤15 kb

Blunt End

SuperMix

AS301

High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR

TransStart® GoldPfu

SuperMix

AS401

54×

2-4 kb/min

≤15 kb

Blunt End

Ultra High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR

TransStart® FastPfu

Enzyme

AP221

54×

4 kb/min

≤15 kb

Blunt End

SuperMix

AS221

Ultra High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR

TransStart® FastPfu Fly

Enzyme

AP231

108×

≤5 kb 12 kb/min;

>5 kb 6 kb/min

≤15 kb

Blunt End

SuperMix

AS231

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