PCR Enzyme & SuperMix Series Product Selection Guide

Application ScenariosSeriesProduct FormCat.No.

Fidelity

(VS EasyTaq®)

Hot-start

Amplification

 Speed

Amplicon Lengt

 (gDNA)

Product End

 Structure

Standard PCRRapid AmplificationTransFast®EnzymeAP1011×-

＜2 kb 1 sec/kb;

≥2kb 10 sec/kb

≤4 kb3'-dA
SuperMixAS102≤6 kb
Standard PCR AmplificationEasyTaq®EnzymeAP1111×-1-2 kb/min≤4 kb3'-dA
SuperMixAS111
Short Fragment Amplification, PAGE DetectionEasyTaq® for PAGEEnzymeAP1121×-1-2 kb/min≤3 kb3'-dA
SuperMixAS112
High-Fidelity PCRComplex Template AmplificationTransTaq® -TEnzymeAP12218×√1-2 kb/min≤8 kb3'-dA
SuperMixAS122
GC/AT-rich template amplification, complex templates, long-fragment PCRTransTaq® HiFiEnzymeAP13118×√1-2 kb/min≤15 kb3'-dA
SuperMixAS131
qPCR, Multiplex PCR, SNP
GC/AT-rich template amplification
Complex template, long-fragment amplification		TransStart® TaqEnzymeAP14118×√1-2 kb/min≤15 kb3'-dA
qPCR, Multiplex PCR, SNP
GC/AT-rich template amplification
Complex template, long-fragment amplification		TransStart® TopTaqEnzymeAP15118×√1-2 kb/min≤15 kb3'-dA
Low-copy template, complex template
qPCR, Multiplex PCR, SNP, GC/AT-rich template amplification		PerfectStart® TaqEnzymeAP40118×√1-2 kb/min≤1 kb3'-dA
Gene Site-Directed MutagenesisTransStart® KD PlusEnzymeAP301108×√1 kb/min≤15 kbBlunt End
SuperMixAS301
High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR		TransStart® GoldPfuSuperMixAS40154×√2-4 kb/min≤15 kbBlunt End
Ultra High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR		TransStart® FastPfuEnzymeAP22154×√4 kb/min≤15 kbBlunt End
SuperMixAS221
Ultra High-Fidelity PCR Rapid Amplification
Gene Site-Directed Mutagenesis
GC/AT-rich template, complex template, long-fragment PCR		TransStart® FastPfu FlyEnzymeAP231108×√

≤5 kb 12 kb/min;

＞5 kb 6 kb/min

≤15 kbBlunt End
SuperMixAS231

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