PCR Enzyme & SuperMix Series Product Selection Guide
Application Scenarios | Series | Product Form | Cat.No. | Fidelity (VS EasyTaq®) | Hot-start | Amplification Speed | Amplicon Lengt (gDNA) | Product End Structure | |
Standard PCR | Rapid Amplification | TransFast® | Enzyme | 1× | - | <2 kb 1 sec/kb; ≥2kb 10 sec/kb | ≤4 kb | 3'-dA | |
SuperMix | ≤6 kb | ||||||||
Standard PCR Amplification | EasyTaq® | Enzyme | 1× | - | 1-2 kb/min | ≤4 kb | 3'-dA | ||
SuperMix | |||||||||
Short Fragment Amplification, PAGE Detection | EasyTaq® for PAGE | Enzyme | 1× | - | 1-2 kb/min | ≤3 kb | 3'-dA | ||
SuperMix | |||||||||
High-Fidelity PCR | Complex Template Amplification | TransTaq® -T | Enzyme | 18× | √ | 1-2 kb/min | ≤8 kb | 3'-dA | |
SuperMix | |||||||||
GC/AT-rich template amplification, complex templates, long-fragment PCR | TransTaq® HiFi | Enzyme | 18× | √ | 1-2 kb/min | ≤15 kb | 3'-dA | ||
SuperMix | |||||||||
qPCR, Multiplex PCR, SNP | TransStart® Taq | Enzyme | 18× | √ | 1-2 kb/min | ≤15 kb | 3'-dA | ||
qPCR, Multiplex PCR, SNP | TransStart® TopTaq | Enzyme | 18× | √ | 1-2 kb/min | ≤15 kb | 3'-dA | ||
Low-copy template, complex template | PerfectStart® Taq | Enzyme | 18× | √ | 1-2 kb/min | ≤1 kb | 3'-dA | ||
Gene Site-Directed Mutagenesis | TransStart® KD Plus | Enzyme | 108× | √ | 1 kb/min | ≤15 kb | Blunt End | ||
SuperMix | |||||||||
High-Fidelity PCR Rapid Amplification | TransStart® GoldPfu | SuperMix | 54× | √ | 2-4 kb/min | ≤15 kb | Blunt End | ||
Ultra High-Fidelity PCR Rapid Amplification | TransStart® FastPfu | Enzyme | 54× | √ | 4 kb/min | ≤15 kb | Blunt End | ||
SuperMix | |||||||||
Ultra High-Fidelity PCR Rapid Amplification | TransStart® FastPfu Fly | Enzyme | 108× | √ | ≤5 kb 12 kb/min; >5 kb 6 kb/min | ≤15 kb | Blunt End | ||
SuperMix | |||||||||