PCR Enzymes
  • AP211-01.JPG
AP211-01.JPG

EasyPfu® DNA Polymerase


Product Details

EasyPfu® DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu® DNA Polymerase possesses a proofreading 3'-5' exonuclease activity.

EasyPfu® DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.

Applications
• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP211-01/11AP211-02/12AP211-03/13
EasyPfu DNA Polymerase250 U×1500 U×1500 U×6
10×EasyPfu Buffer1.2 ml ×11.2 ml ×11.2 ml ×6
2.5 mM dNTPs- / 500 μl ×1- / 1 ml ×1- / 1 ml ×6
6×DNA Loading Buffer500 μl×11 ml ×11 ml ×2
50 mM MgSO4200 μl×1400 μl×11 ml ×1


Citations

Zhao J , Liu Q , Hu P , et al. An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana[J]. Scientific Reports, 2016, 6(1):20573.

Ding K , Pei T , Bai Z , et al. SmMYB36, a Novel R2R3-MYB Transcription Factor, Enhances Tanshinone Accumulation and Decreases Phenolic Acid Content in Salvia miltiorrhiza Hairy Roots[J]. Scientific Reports, 2017, 7(1):5104

Guo X , Fan C , Chen Y , et al. Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 ( DGAT1 ) gene from the microalga Chlorella ellipsoidea[J]. Bmc Plant Biology, 2017, 17(1):48.

Meng D, Shen L, Yang R, et al. Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus[J].Biochim Biophys Acta. 2014, 1840(1):120-8.

Zheng T , Huang Q , Zhang C , et al. Development of a Simvastatin Selection Marker for a Hyperthermophilic Acidophile, Sulfolobus islandicus[J]. Applied and Environmental Microbiology, 2012, 78(2):568-574.

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