EasyPfu® DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu® DNA Polymerase possesses a proofreading 3'-5' exonuclease activity.
• EasyPfu® DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.
Applications
• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
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Guo X , Fan C , Chen Y , et al. Identification and characterization of an efficient acyl-CoA: diacylglycerol acyltransferase 1 ( DGAT1 ) gene from the microalga Chlorella ellipsoidea[J]. Bmc Plant Biology, 2017, 17(1):48.
Meng D, Shen L, Yang R, et al. Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus[J].Biochim Biophys Acta. 2014, 1840(1):120-8.
Zheng T , Huang Q , Zhang C , et al. Development of a Simvastatin Selection Marker for a Hyperthermophilic Acidophile, Sulfolobus islandicus[J]. Applied and Environmental Microbiology, 2012, 78(2):568-574.
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Stem Cell Related
Antibodies
Instruments
SARS-CoV-2 Detection
