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TransNGS® Library Quantification Kit for Illumina®


Product Details

TransNGS® Library Quantification Kit for Illumina® provides all reagents needed for qPCR based quantification of DNA libraries prepared for Illumina next generation sequencing platforms. Five linear DNA Standards with different GC contents are provided for customers to choose from. The kit also contains PCR primers which target the P5 and P7 adaptor sequences and TransNGS® Library Quantification qPCR SuperMix (2×) for library amplifications.

Five sets of linear DNA Standards (25%, 37.5%, 50%, 62.5%, and 75% of GC contents) to choose from Each DNA Standard contains six 10-fold dilution series of a linear 420 bp template TransNGS® Library Quantification qPCR SuperMix (2×) enables accurate library amplification with various GC contents.

Applications
Quantification of DNA libraries prepared for Illumina next generation sequencing platforms with P5 and P7 adaptor sequences.

Storage
at -20℃ in dark for one year.

Shipping
Dry ice (-70℃). 

Product Contents


ComponentKQ101-01 (100 rxns)KQ101-02 (500 rxns)
TransNGS® Library Quantification qPCR SuperMix (2×)1 ml5×1 ml
Library Quantification Primer Mix (20×)100 μl500 μl
DNA Standards (S1-S6)24 μl each120 μl each
Library Dilution Buffer (10×)1 ml5 ml
Passive Reference Dye (50×)40 μl200 μl
Nuclease-free Water10 ml50 ml


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