Viral nucleic acid extraction is imperative in delivering intact, un-damaged and contamination-free starting materials for the very sensitive method of polymerase chain reaction (PCR) detection of virus DNA/RNA. 3 effective methods are recommended here for viral nucleic acid extraction:
Manual spin column-based nucleic acid extraction
EasyPure® Viral DNA/RNA Kit (Cat. No. ER201)
This kit utilizes a unique lysis buffer to lyse virus and a silica-based spin column to specifically bind to DNA/RNA showing simple procedures and high yield.
Manual magnetic beads nucleic acid extraction
MagicPure® Viral DNA/RNA Kit (Cat. No. EC301)
This kit uses silica magnetic beads to specifically bind to DNA/RNA, which will be pelleted by a magnetic stand for separation. It shows high yield and high purity with simple and fast procedures.
Automated nucleic acid extraction
(1) Pre-filled 32-well nucleic acid extraction kit: MagicPure® 32 Viral DNA/RNA Kit (Cat. No. EC301-32)
This kit can be used directly on a 32-channel magnetic-rob automated nucleic acid extraction instrument.
Application example:qPCR amplification curves for PRV DNA extracted from 32 repeated samples of 200 μl of cell supernatant using MagicPure® 32 Viral DNA/RNA Kit (Cat. No. EC301-32).
(2) Pre-filled 96-well nucleic acid extraction kit:MagicPure® 96 Viral DNA/RNA Kit (Cat. No. EC301-96)
This kit can be used directly on a 96-channel magnetic-rob automated nucleic acid extraction instrument.
Application example: qPCR amplification curves for PRV DNA extracted from 96 repeated samples of 200 μl of cell supernatant using MagicPure® 96 Viral DNA/RNA Kit (Cat. No. EC301-96).
Product summary for viral nucleic acid extraction
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