Mutagenesis Systems
  • FM111-01.JPG
FM111-01.JPG

Fast Mutagenesis System


Product Details

• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.

• Mutation sites on both primers to improve mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.

Storage
Competent Cell at -70°C for six months; others at -20°C for two years

Shipping
Dry ice (-70℃)

Product Contents


ComponentFM111-01 (10 rxns)FM111-02 (20 rxns)
TransStart® FastPfu Fly PCR SuperMix250 μl500 μl
DMT Enzyme (10 units/μl)10 μl20 μl
DMT Chemically Competent Cell10×50 μl20×50 μl
Nuclease-free Water1 ml1 ml
SControl Plasmid (5 ng/μl)10 μl20 μl
SControl Primers (10 μM )10 μl20 μl


Citations

Wang J, Zhou L, Shi H, et al. A single transcription factor promotes both yield and immunity in rice[J].Science, 2018, 7, 361(6406):1026-1028.

Li W , Zhu Z , Chern M , et al. A Natural Allele of a Transcription Factor in Rice Confers Broad-Spectrum Blast Resistance[J]. Cell, 2017, 170(1):114-126.e15.

Zhou X, Wang B, Pan Q, et al. Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history[J]. Nature Genetics, 2014, 46(12):1303-1310.

Zhang H , Lin X , Han Z , et al. Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs[J]. Cell Research, 2016, 26(5):543-55. 

Song N , Liu Z S , Xue W , et al. NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation[J]. Molecular Cell, 2017, 68(1):185-197.


Message