Mutagenesis Systems
  • FM111-01.JPG

Fast Mutagenesis System

Product Details

• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.
• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.

• Mutation sites on both primers to improve mutation efficiency.
• Partially overlapping primers for exponential DNA amplification.
• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.
• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.

Competent Cell at -70°C for six months; others at -20°C for two years

Dry ice (-70℃)

Product Contents

ComponentFM111-01 (10 rxns)FM111-02 (20 rxns)
TransStart® FastPfu Fly PCR SuperMix250 μl500 μl
DMT Enzyme (10 units/μl)10 μl20 μl
DMT Chemically Competent Cell10×50 μl20×50 μl
Nuclease-free Water1 ml1 ml
SControl Plasmid (5 ng/μl)10 μl20 μl
SControl Primers (10 μM )10 μl20 μl


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