Product Details
This product uses methylated plasmid as the template with partially overlapping primers (both containing the mutation) and
2× TransStart® FastPfu Fly PCR SuperMix for amplification. The PCR products are subjected to in vitro digestion with
DMT enzyme and in vitro degradation by DMT Chemically Competent Cells to remove the non‑mutated parental plasmid.
The system features high mutation efficiency and a simple workflow.
Storage
DMT Chemically Competent Cell at -70℃ or below for one year, others at -18℃ or below for two years
Shipping
Dry ice (-70℃)
| Component | FM111-01 (10 rxns) | FM111-02 (20 rxns) |
| 2×TransStart® FastPfu Fly PCR SuperMix | 250 μl | 500 μl |
| DMT Enzyme (10 units/μl) | 10 μl | 20 μl |
| DMT Chemically Competent Cell | 10×50 μl | 20×50 μl |
| Nuclease-free Water | 1 ml | 1 ml |
| SControl Plasmid (5 ng/μl) | 10 μl | 20 μl |
| SControl Primers (10 μM ) | 10 μl | 20 μl |
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