Mutagenesis Systems
  • FM201-01.JPG
FM201-01.JPG

Fast MultiSite Mutagenesis System


Product Details

Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.

• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.

Notes
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.

Storage
at -20°C for two years

Shipping
Dry ice (-70℃)

Product Contents


ComponentFM201-01 (10 rxns)
TransStart® FastPfu Fly PCR SuperMix1 ml
DMT Enzyme (10 units/μl)30 μl
2×Assembly Mix50 μl
DMT Chemically Competent Cell10×50 μl
Nuclease-free Water1 ml


Citations

Rudian Z , Yibin Z , Xiaojing P , et al. Regulation of Antimicrobial Peptides in Aedes aegypti Aag2 Cells[J]. Frontiers in Cellular and Infection Microbiology, 2017, 7:22.

Du G , Gu Y , Hao C , et al. The cellular distribution of Na + /H +, exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer[J]. Oncotarget, 2016, 7(20):29440-29453.

Chen Y , Luo Q , Zhou W , et al. Improving the catalytic efficiency ofBacillus pumilusCotA-laccase by site-directed mutagenesis[J]. Applied Microbiology and Biotechnology, 2017, 101(5):1935-1944.

Zeng F M , Wang X N , Shi H S , et al. Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior[J]. Amino Acids, 2017, 49(5):943-955.

Lu Y X , Liu X X , Liu W B , et al. Identification and characterization of Two types of Amino Acid-regulated acetyltransferases in Actinobacteria[J]. Bioscience Reports, 2017, 37(4):BSR20170157.


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