Product Details
This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene. It recognizes and cleaves
non-perfectly matched DNA, cruciform DNA structures, Holliday junctions, heteroduplex DNA, and randomly cleaves
single‑stranded DNA. The cleavage site is at the first, second, or third phosphodiester bond that is 5´ to the mismatch.
Applications
• Gene mutation and SNP detection for result from TALEN and CRISPR/CAS9
• Recognition and cleavage for non-perfectly matched DNA and Holliday junctions
• Randomly cleave single-stranded DNA
Storage
at -20oC for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | LE101-01 | LE101-02 |
| T7 Endonuclease I | 250 units | 5×250 units |
| 10×T7 Endonuclease I Buffer | 200 μl | 1 ml |
| T7 Endonuclease I Control Template (40 ng/μl) | 20 μl | 20 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |
