This product is purified from E. coli after inducible expression of a recombinant vector containing Poly (A) Polymerase gene. It has a molecular weight of 50.2 kDa. The reaction of E. coli Poly (A) Polymerase is template-independent. It catalyzes the addition of AMP converted from ATP to the 3'end of the RNA, that is, adding a poly (A) tail to the RNA.
Highlights
Template independent addition of a poly (A) tail to the 3'end of the RNA.
Application
Add a poly(A) tail for reverse transcription into cDNA; labeling RNA with modified ATP or its analogues.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)