Modification Enzymes
  • FL101-01.JPG
FL101-01.JPG

T4 DNA Ligase


Product Details

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive end. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-strand nucleic acids. T4 DNA Ligase requires ATP as a cofactor.

Concentration
200 units/μl

Unit Definition
One unit is the amount of enzyme required to give 50% ligation of Hind III fragments of λDNA (5´ DNA termini concentration of 0.12 μM, 200 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1×T4 DNA Ligase Buffer.

Storage
at -20°C  for one year

Shipping
Dry ice (-70 ℃)

References

1.Xiao C C , Zhang J , Luo P C , et al. Identification of Tisp40 as an Essential Regulator of Renal Tubulointerstitial Fibrosis via TGF-β/Smads Pathway[J]. Cellular Physiology and Biochemistry, 2017:697-712.

2.Wang T N , Zhao M . A simple strategy for extracellular production of CotA laccase in Escherichia coli, and decolorization of simulated textile effluent by recombinant laccase[J]. Applied Microbiology and Biotechnology, 2017, 101(2):685-696.

3.Wang C , Zhang H B , Li M Q , et al. Functional analysis of truncated and site-directed mutagenesis dextransucrases to produce different type dextrans[J]. Enzyme and Microbial Technology, 2017, 102:26-34.

4.Wang X , Lei G , Wu X , et al. Expression, purification and characterization of sll1981 protein from cyanobacterium Synechocystis sp. PCC6803[J]. Protein Expression & Purification, 2017, 139:21-28.


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