Modification Enzymes
  • GD111.JPG
GD111.JPG

DMT Enzyme


Product Details

DMT cuts the sequence GmATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.

Unit Definition
One unit (U) is defined as the amount of enzyme required for completely digesting 1 μg methylated pBR322 DNA at 37°C for one-hour reaction in a 50 μl reaction system.

Applications
In vitro site-directed DNA mutagenesis; degradation of methylated plasmid template

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

References

1.Liu M , Feng Z , Ke H , et al. Tango1 spatially organizes ER exit sites to control ER export[J]. The Journal of Cell Biology, 2017, 216(4):1035-1049.

2.Bingqing L , Yingying Y , Yajie Z , et al. A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity[J]. Frontiers in Cellular and Infection Microbiology, 2017, 7:26.

3.Wang T N , Zhao M . A simple strategy for extracellular production of CotA laccase in Escherichia coli, and decolorization of simulated textile effluent by recombinant laccase[J]. Applied Microbiology and Biotechnology, 2017, 101(2):685-696.


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