Product Details
Thermostable RNase HII is an endoribonuclease derived from Pyrococcus abyssi (P.a.), genetically engineered and expressed in Escherichia coli (E. coli). This enzyme cleaves the 5' phosphodiester of ribonucleotides within the context of dsDNA, generating
5' phosphate and 3' hydroxyl ends. It exhibits very low activity against single-stranded RNA (ssRNA) and no cleavage activity against double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA). The enzyme is highly thermostable, retaining its
activity even after heating at 95℃ for 45 minutes. It is widely used in applications such as RNase HII-dependent PCR (rhPCR), loop-mediated isothermal amplification (LAMP), and degradation of the RNA portion of Okazaki fragments.
at -18℃ or below for two years
Dry ice (-70℃)
