Expression Competent Cells
  • CD701-01.JPG
CD701-01.JPG

BL21(DE3) pLysS Chemically Competent Cell


Product Details

BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F- ompT hsdS(rB-mB-) gal dcm(DE3) pLysS CamR

Features
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• CamR.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein's expression.
• Control plasmid III (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

Storage
at -70℃ for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70℃). 

Citations

Zhang C, Ye BC,et al.  A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell. Biosensors and Bioelectronics,2014;54:15-9.

Yao W , Kang L , Gao S , et al. Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin[J]. Toxicon, 2015, 100:46-52.

Dang W , Lu H , Gao Y , et al. Molecular analysis of inducible Heat shock protein 70 of Pelodiscus sinensis and its effects during pathogen (Aeromonas hydrophila) infection[J]. Aquaculture, 2015, 442:93-99.

Shi Y , Li Y , Lei Y , et al. A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11[J]. Journal of Virology, 2016, 90(9):JVI.03065-15.

Zhang C, Wei ZH, Ye BC , et al.Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor. Appl Microbiol Biotechnol,2013 ,97(18):8307-16. 



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