Expression Competent Cells
  • CD601-01.JPG
CD601-01.JPG

BL21(DE3) Chemically Competent Cell


Product Details

BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F- ompT hsdS(rB-mB-) gal dcm(DE3)

Features
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• DE3 strains contains the λDE3 lysogen that carries the gene for T7 RNA polymerase.
• Suitable for T7 and T7lac such as pET, pEASY®.
• Suitable for high expression of non-toxic protein.
• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

Storage
at -70℃ for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70℃)

Citations

Zhao FL, Zhang C, Zhang C, et al. A genetically encoded biosensor for in vitro and in vivo detection of NADP+[J]. Biosensors and Bioelectronics, 2016, 77:901-906.

Liu K , Shen D , Shen J , et al. The Super Elongation Complex Drives Neural Stem Cell Fate Commitment[J]. Developmental Cell, 2017, 40(6):537-551.e6.

Liu S , Yu H , Liu Y , et al. Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis[J]. Molecular Cell, 2017:S1097276517305051.

Hu J, Yang H, Mu J, et al. Nitric Oxide Regulates Protein Methylation during Stress Responses in Plants

Wang X, Xuan Y, Han Y,et al. Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting.cell,2019,176(3):625-635.e14.


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