ATAC

KP171

Illumina platform

10-50000 mammalian cells

• Low cell input: Requires as few as 10–50,000 cells to obtain high-resolution sequencing profiles
• Short library prep time: For 5,000 cells or fewer, library prep can be completed in under 2 hours
• High library yield: High-quality library with good peak profile
• Good library peak profile: Library peaks at 200–2000 bp, no significant adapter peaks
• No size selection needed: Size selection is not required unless there are specific requirements for library fragment length distribution

Chromatin accessibility / open chromatin studies

Cut&Tag

KP172

Illumina platform

10-10⁵ mammalian cells or processed nuclei, and eukaryotic cells without cell wall structures (e.g., plant cell protoplasts)

• Short operation time: Library construction can be completed in approximately 5.5 hours
• High-quality sequencing data: High library yield, good library peak profile
• Accurate and reliable data: High clean data percentage, more stable mapping rate, accurate and effective peak calling
• Good antibody compatibility and high affinity

Protein-DNA interaction

Antibody

HS001

Goat anti-rabbit antibody, used as a secondary antibody with the CUT&Tag Library Prep Kit (KP172) to bridge the primary antibody and Tn5 to form an immune complex

Low background, high sensitivity

CUT&Tag: Recommended 1:100 dilution, final concentration 10 μg/ml

HS002

Goat anti-mouse antibody, used as a secondary antibody with the CUT&Tag Library Prep Kit (KP172) to bridge the primary antibody and Tn5 to form an immune complex