PCR Enzymes
  • AP122-01.JPG
AP122-01.JPG

TransTaq®-T DNA Polymerase


Product Details

TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.

TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

Applications
• Complex templates
• TA cloning

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAP122-01/11AP122-02/12AP122-03/13
TransTaq®-T DNA Polymerase250 U×1500 U×1500 U×6
10×TransTaq®1.2 ml ×11.2 ml ×11.2 ml ×6
2.5 mM dNTPs- / 400 μl ×1- / 800 μl ×1- / 800 μl ×6
6×DNA Loading Buffer500 μl×11 ml ×11 ml ×2


References

Zhang Y , Ji G , Wang R . Drivers of nitrous oxide accumulation in denitrification biofilters with low carbon:nitrogen ratios[J]. Water Research, 2016:S0043135416307230.

Yuwen L , Zhang FL , Chen QH , et al. The role of aromatic L-amino acid decarboxylase in bacillamide C biosynthesis by Bacillus atrophaeus C89[J]. Scientific Reports, 2013, 3.

Zhang Y , Ji G , Wang R . Genetic associations as indices of nitrogen cycling rates in an aerobic denitrification biofilter used for groundwater remediation[J]. Bioresource Technology, 2015, 194:49-56.

Liu G , Zhou L , Zhang L , et al. The Complete Mitochondrial Genome of Bean Goose (Anser fabalis) and Implications for Anseriformes Taxonomy[J]. PLOS ONE, 2013, 8.

Wu R , Liu Q , Zhang P , et al. Tandem amino acid repeats in the green anole (Anolis carolinensis) and other squamates may have a role in increasing genetic variability[J]. BMC Genomics, 2016, 17(1):109.

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