Direct PCR Product Selection Guide

Application ScenariosCat.No.Applicable Sample TypesSample AmountAmplicon LengthMultiplex Amplification
Mammalian tissue direct amplificationAD201Cultured mammalian cells, blood, saliva, hair, animal tissue (mammalian, marine animals, insects), etc.Animal cells ≤10cells
Hair ≤10 mg
Animal tissue ≤10 mg
Mouse tail ≤0.5 cm
Mouse ear ≤0.5 cm2
Saliva ≤10 μl
Blood ≤10 μl
≤3 kbCells: 6-plex
Tissues: 5-plex
Plant tissue direct amplificationAD301Non-polysaccharide, non-polyphenol plantsPlant tissue: 5 mg or about 0.5 cm2≤2 kb5-plex
Blood direct amplificationAD401EDTA, heparin, sodium citrate anticoagulant methods stored fresh or frozen blood samples; blood samples without anticoagulant and dried blood; human oral epithelial cells

Human blood: 0.5-2 μl

Mouse blood: 1/20-1/80 dilution then add 1 μl

Avian blood: 1/1-1/100 dilution then add 1 μl

Cultured cells: 10-10cells

Epithelial cells: 0.5-5 μl

Dried blood: 0.5-5 μl;

   Hole diameter 1 mm: 1-8

   Hole diameter 2 mm: 1-2
FTA blood card: 

   Hole diameter 0.5 mm: 1-8

   Hole diameter 1 mm: 1-2

≤4 kb-
Mouse direct amplificationAD501Ears, toes, tails (fresh or frozen stored) etc.Mouse ear: punch a 3mm diameter hole or cut a piece of similar size;
Mouse toe: about 2mm long toe piece;
Mouse tail: about 2mm tail tip
≤3 kb5-plex

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