Direct PCR Product Selection Guide
Application Scenarios | Cat.No. | Applicable Sample Types | Sample Amount | Amplicon Length | Multiplex Amplification |
Mammalian tissue direct amplification | Cultured mammalian cells, blood, saliva, hair, animal tissue (mammalian, marine animals, insects), etc. | Animal cells ≤106 cells | ≤3 kb | Cells: 6-plex | |
Plant tissue direct amplification | Non-polysaccharide, non-polyphenol plants | Plant tissue: 5 mg or about 0.5 cm2 | ≤2 kb | 5-plex | |
Blood direct amplification | EDTA, heparin, sodium citrate anticoagulant methods stored fresh or frozen blood samples; blood samples without anticoagulant and dried blood; human oral epithelial cells | Human blood: 0.5-2 μl Mouse blood: 1/20-1/80 dilution then add 1 μl Avian blood: 1/1-1/100 dilution then add 1 μl Cultured cells: 10-105 cells Epithelial cells: 0.5-5 μl Dried blood: 0.5-5 μl； Hole diameter 1 mm: 1-8 Hole diameter 2 mm: 1-2 Hole diameter 0.5 mm: 1-8 Hole diameter 1 mm: 1-2 | ≤4 kb | - | |
Mouse direct amplification | Ears, toes, tails (fresh or frozen stored) etc. | Mouse ear: punch a 3mm diameter hole or cut a piece of similar size; | ≤3 kb | 5-plex |
