pEASY®-T5 Zero Cloning Kit
Catalog Number： CT501-01
Price：Please inquire first
Product Details
This product contains a suicide gene. When the insert is successfully connected to the vector, the expression of the suicide gene will be disrupted, and the host cell can grow normally, otherwise the host cell will die, thus realizing the "Zero" background. It is suitable for TA cloning.
• Fast: 5 minutes only.
• Easy: Only adding the insert is needed.
• Highly efficient: Almost 100% high positive rate.
• Low background: Close to zero.
• No blue/white selection needed.
• Suitable for short and large fragment cloning.
• Kanamycin and Ampicillin resistance genes are convenient for selection according to experimental conditions.
• M13 forward primer and M13 reverse primer for sequencing.
• T3 promoter and T7 promoter for in vitro transcription.
• Trans1-T1 Phage Resistant Chemically Competent Cell features high transformation efficiency and fast growth, generating
sufficient colonies to save time.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70oC or below for one year; others at -18℃ or below for one year.
Shipping
Dry ice (-70℃)
| Component | CT501-01 (20 rxns) | CT501-02 (60 rxns) |
| pEASY ®-T5 Zero Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
1 Chen W, Chen L, Zhang X, et al. Convergent selection of a WD40 protein that enhances grain yield in maize and rice[J]. Science, 2022.(IF 63.71)
2 Zhao Z, Zhang Z, Li J, et al. Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness[J]. Elife, 2020.(IF 7.08)
