pEASY Cloning Vectors
  • CT501-01.JPG
CT501-01.JPG

pEASY® -T5 Zero Cloning Kit


Product Details

pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.

•5 minutes fast ligation of Taq-amplified PCR products.
•High cloning efficiency. Positive clones up to 100%.
•No blue/white selection needed.
•Suitable for short and large fragment cloning.
•Kanamycin and Ampicillin resistance genes for selection.
•M13 forward primer and M13 reverse primer for sequencing.
•T3 promoter and T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCT501-01 (20 rxns)CT501-02 (60 rxns)
pEASY ®-T5 Zero Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl



Citations

Xia B , Han D , Lu X , et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale[J]. Nature Methods, 2015, 12(11):1047-50.

Gao Y , Chen J , Li K , et al. Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming[J]. Cell Stem Cell, 2013, 12(4):453-469.

Le R , Kou Z , Jiang Y , et al. Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem Cells[J]. Cell Stem Cell, 2014, 14(1):27-39.

Gao S , Zheng C , Chang G , et al. Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cells[J]. Nature Communications, 2015, 6:6318.

High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells[J]. Cell Research, 2014, 24(3):293-306.


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