pEASY Cloning Vectors
  • CT111-01.JPG
CT111-01.JPG

pEASY® -T1 Simple Cloning Kit


Product Details

pEASY®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•SR primer and M13 forward primer for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCT111-01 (20 rxns)CT111-02 (60 rxns)
pEASY ®-T1 Simple Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
SR Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl


Citations

Liu Q , Liu H , Gong Y , et al. An Atypical Thioredoxin Imparts Early Resistance to, Sugarcane Mosaic Virus, in Maize[J]. Molecular Plant, 2017, 10(3):483-497.

Fang L , Ge H , Huang X , et al. Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light-Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803[J]. Molecular Plant, 2016, 10(1):73-85.

Li J , Wang T , Shao B , et al. Plasmid-Mediated Quinolone Resistance Genes and Antibiotic Residues in Wastewater and Soil Adjacent to Swine Feedlots: Potential Transfer to Agricultural Lands[J]. Environmental Health Perspectives, 2012, 120(8):1144-1149.

Luo J , Liang S , Li J , et al. A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double-stranded RNA homologous to fatty acyl-coenzyme A reductase in cotton.[J]. New Phytologist, 2017, 215(3):1173-1185. 

Wang C , Yang Q , Wang W , et al. A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maize[J]. New Phytologist, 2017, 215(4):1503. 


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