pEASY Cloning Vectors
  • CT101-01.JPG
CT101-01.JPG

pEASY® -T1 Cloning Kit


Product Details

pEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCT101-01 (20 rxns)CT101-02 (60 rxns)
pEASY ®-T1 Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl



Citations

Zuo W , Chao Q , Zhang N , et al. A maize wall-associated kinase confers quantitative resistance to head smut[J]. Nature Genetics, 2015, 47(2):151-157.

Sun W , Shen Y H , Han M J , et al. An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx mori[J]. Molecular Biology and Evolution, 2014, 31(12):3302-3313.

Xiang X , Huang X , Wang H , et al. pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements[J]. Life, 2015, 5(1):506-520.

Guo Z , Kang S , Zhu X , et al. The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management[J]. Scientific Reports, 2015, 5(1):13728.

Wang X , He G , Mai K , et al. Differential regulation of taurine biosynthesis in rainbow trout and Japanese flounder[J]. Scientific Reports, 2016, 6:21231.


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