pEASY®-T1 Cloning Kit
Catalog Number： CT101-01
Price：Please inquire first
Product Details
The pEASY®-T1 Cloning Vector contains the LacZ gene, enabling blue-white screening on plate media with IPTG and X-gal, making it suitable for TA cloning.
Features
Fast: Takes only 5 minutes.
Simple: Just add the fragment.
Efficient: High positive rate.
Provides two selection markers, ampicillin and kanamycin, allowing flexible selection based on experimental needs.
Multiple restriction enzyme sites for convenient subcloning.
Sequencing primers: M13 Forward Primer, M13 Reverse Primer, T7 Promoter Primer.
T7 Promoter for in vitro transcription.
Trans1-T1 competent cells offer high transformation efficiency and fast growth, ensuring high clone yield and reducing screening time.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70℃ or below for one year; others at -18℃ or below for one year.
Shipping
Dry ice (-70℃)
| Component | CT101-01 (20 rxns) | CT101-02 (60 rxns) |
| pEASY ®-T1 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
1 Lu S, Luo S, Liu C, et al. Induction of significant neutralizing antibodies against SARS-CoV-2 by a highly attenuated pangolin coronavirus variant with a 104nt deletion at the 3'-UTR[J]. Emerging Microbes & Infections, 2023.(IF 19.56)
2 Li Y, Xu J, Guo X, et al. The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model[J]. Genome Biology, 2023.(IF 17.90)
3 Shi Y, Xu M, Duan X, et al. WarmStart colorimetric loop-mediated isothermal amplification for the one-tube, contamination-free and visualization detection of Shigella flexneri[J]. International Journal of Infectious Diseases, 2021.(IF 12.07)
4 Wang S, Teng D, Li X, et al. The evolution and diversification of oakleaf butterflies[J]. Cell, 2022.(IF 66.85)
5 Zhang Q, Zhang X, Zhu Y, et al. Recognition of cyclic dinucleotides and folates by human SLC19A1[J]. Nature, 2022.(IF 69.50)
6 Xia J, Guo Z, Yang Z, et al. Whitefly hijacks a plant detoxification gene that neutralizes plant toxins[J]. Cell, 2021.(IF 38.63)
7 Liu Q, Deng S, Liu B, et al. A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease[J]. Nature Communications, 2020.(IF 12.12)
