pEASY Cloning Vectors
  • CB111-01.JPG
CB111-01.JPG

pEASY® -Blunt Simple Cloning Kit


Product Details

pEASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•SR primer and M13 forward primer for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months


Shipping
Dry ice (-70℃)

Product Contents


ComponentCB111-01 (20 rxns)CB111-02 (60 rxns)
pEASY ®-Blunt Simple Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
SR Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl


Citations

Weiqi Z , Haifeng W , Guihai F , et al. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys[J]. Nature, 2018,560(7720):661-665. 

Qi J , Wu B , Feng S , et al. Mechanical regulation of organ asymmetry in leaves[J]. Nat Plants, 2017, 3(9):724-733.

Liu Q , Ning Y , Zhang Y , et al. OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice[J]. The Plant Cell, 2017, 29(2):345-359.

Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.

Yu Z , Zhang B , Sun W , et al. Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis[J]. Fungal Diversity, 2013, 58(1):127-141.


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