pEASY®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.
•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70℃)
Wang Y , Cheng X , Shan Q , et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew[J]. Nature Biotechnology, 2014, 32(9):947-951.
Xie ZX, Li BZ, Mitchell LA, et al. “Perfect”designer chromosome V and behavior of a ring derivative[J]. Science, 2017,10, 55(6329).
Zhou F , Lin Q , Zhu L , et al. Corrigendum: D14–SCFD3-dependent degradation of D53 regulates strigolactone signalling[J]. Nature, 2016 ,21, 532(7599):402.
Shan Q , Wang Y , Li J , et al. Targeted genome modification of crop plants using a CRISPR-Cas system[J]. Nature Biotechnology, 2013, 31(8):686-688.
Ye J , Ge J , Zhang X , et al. Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds[J]. Cell Research, 2016, 26(1):34-45.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
