pEASY Cloning Vectors
  • CB101-01.JPG
CB101-01.JPG

pEASY® -Blunt Cloning Kit


Product Details

pEASY®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months

Shipping
Dry ice (-70℃)

Product Contents


ComponentCB101-01 (20 rxns)CB101-02 (60 rxns)
pEASY ®-Blunt Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl



Citations

Wang Y , Cheng X , Shan Q , et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew[J]. Nature Biotechnology, 2014, 32(9):947-951.

Xie ZX, Li BZ, Mitchell LA, et al. “Perfect”designer chromosome V and behavior of a ring derivative[J]. Science, 2017,10, 55(6329).

Zhou F , Lin Q , Zhu L , et al. Corrigendum: D14–SCFD3-dependent degradation of D53 regulates strigolactone signalling[J]. Nature, 2016 ,21, 532(7599):402.

Shan Q , Wang Y , Li J , et al. Targeted genome modification of crop plants using a CRISPR-Cas system[J]. Nature Biotechnology, 2013, 31(8):686-688.

Ye J , Ge J , Zhang X , et al. Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds[J]. Cell Research,  2016, 26(1):34-45.


Message