pEASY Cloning Vectors
  • CB101-01.JPG

pEASY® -Blunt Cloning Kit

Product Details

pEASY®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Competent Cell at -70°C for six months; others at -20°C for 9 months

Dry ice (-70℃)

Product Contents

ComponentCB101-01 (20 rxns)CB101-02 (60 rxns)
pEASY ®-Blunt Cloning Vector (10 ng/μl)20 μl3×20 μl
Control Template (5 ng/μl)5 μl5 μl
Control Primers (10 μM)5 μl5 μl
M13 Forward Primer (10 μM)50 μl150 μl
M13 Reverse Primer (10 μM)50 μl150 μl
Trans1-T1 Phage Resistant Chemically Competent Cells10×100 μl30×100 μl


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