Cloning Competent Cells
  • CD101-01.JPG
CD101-01.JPG

Trans10 Chemically Competent Cell


Product Details

Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to StrR.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG

Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• StrR.
• Blue/white selection.
• Toxic gene cloning and stable replication of plasmid DNA.

Storage
at -70 °C for six months

Shipping
Dry ice (-70℃)

Citations

Wang X1, Xuan Y1, Han Y, er al. Regulation of HIV-1 Gag-Pol expression by Shiftless, an inhibitor of programmed -1 ribosomal frameshifting[J]. Cell, 2019, 24, 176(3):625-635.

Wang Y , Shi M , Feng H , et al. Structural Insights into Non-canonical Ubiquitination Catalyzed by SidE[J]. Cell, 2018,17, 173(5):1231-1243.

Guo Z , Kang S , Chen D , et al. MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth[J]. PLOS Genetics, 2015, 11(4):e1005124.

Chen X , Xu F , Zhu C , et al. Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans[J]. Scientific Reports, 2014, 4:7581.

Dai Z , Wang B , Liu Y , et al. Producing aglycons of ginsenosides in bakers' yeast[J]. Scientific Reports, 2014, 15(4):3698.


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