This product contains Bst II DNA Polymerase, Reverse Transcriptase (High Temperature), 2.5× pH Sensitive LAMP Reaction Mix, and visual pH-sensitive indicator N-Red. Only template and primers need to be prepared by the user. This product is suitable for performing visual LAMP/RT-LAMP reactions using DNA/RNA as templates.

Bst II DNA Polymerase is a recombinant Bacillus stearothermophilus DNA polymerase, purified after expression in Escherichia coli. This enzyme exhibits 5’→3’ DNA polymerase activity and lacks 5’→3’ exonuclease activity. Reverse Transcriptase (High Temperature) is a high-temperature-tolerant reverse transcriptase, which, when used in combination with

Bst II, allows for one-step reverse transcription and LAMP amplification using RNA as a template at a constant temperature of 65°C. 2.5× pH Sensitive LAMP Reaction Mix is an optimized pre-mixed reaction solution containing necessary components for LAMP amplification, including MgSO₄ and dNTPs. No additional components need to be added during system preparation. N-Red Stain is a pH-sensitive indicator that changes color within the pH range of 6.8 to 8.0. By adding the N-Red color  indicator to the LAMP reaction mixture in advance, there is no need to open the lid after the reaction. Visual observation of the color results allows for distinguishing between positive (yellow) and negative (red) reaction wells.

Features

• Isothermal amplification (LAMP/RT-LAMP) capability

• Rapid polymerization

• Strong strand displacement ability

Application 

• Isothermal amplification of DNA/RNA

• Nucleic acid sequencing in GC-rich regions

• Suitable for experiments requiring strand displacement at high temperatures

Storage

at -18℃ or below for two years