One-Stop Efficient Solutions For Exosome Research and Application

A New Focus in Life Science and Medical Research

Exosomes are nano-sized vesicles (approximately 30-150 nm in diameter) actively secreted by cells. They carry a variety of biomolecules from their parent cells, including proteins, lipids, and nucleic acids (mRNA, miRNA,lncRNA, DNA, etc.) and play key roles in intercellular communication, immune regulation, tumor microenvironment remodeling, tissue regeneration and repair. In recent years, exosome research has rapidly evolved from the initial concept of "cellular garbage" to a frontier hotspot in life sciences and translational medicine. Their applications span the following three major areas:

Basic Research: Exploring the molecular mechanisms of exosomes in physiology (e.g., neural development, immune regulation) and pathology (e.g., tumor metastasis, neurodegenerative diseases, cardiovascular diseases); deciphering the regulatory networks of exosome biogenesis; identifying novel exosome markers.

Biomarker Development: Utilizing the nucleic acid and protein profiles of exosomes in body fluids (blood, urine, cerebrospinal fluid, saliva, etc.) for non-invasive or minimally invasive early disease diagnosis, treatment monitoring, and prognosis assessment.

Translational & Therapeutic Applications: Developing "cell-free" therapies based on exosomes derived from mesenchymal stem cells or other cell types for repairing myocardial infarction, promoting nerve regeneration, treating acute kidney injury and enhancing wound healing; engineering exosomes as endogenous drug delivery vehicles to efficiently load small molecules, nucleic acid drugs or CRISPR components, bypassing biological barriers for precision medicine; advancing clinical-grade exosome preparation and GMP production to propel exosome drugs into clinical trials and applications.

Despite the promising prospects of exosome research, researchers and industry professionals often encounter three major bottlenecks in practice:

Inconsistent Cell Culture Quality: Different culture conditions (presence or absence of serum, media formulation, GMP grade) directly affect exosome yield, composition and function, leading to irreproducible experimental results. 

Inefficient and Harsh Isolation/Purification: Traditional ultracentrifugation is time-consuming, equipment-dependent and the shear force can easily damage exosome structures; some commercial kits suffer from insufficient purity or batch-to-batch variability.

Difficulty in Downstream Nucleic Acid Analysis: Small RNA abundance within exosomes is extremely low and prone to degradation. Conventional extraction methods often fail to obtain RNA of sufficient purity, impacting downstream applications like qPCR and sequencing.

To address these pain points, we provide a standardized, high-performance product portfolio covering “Cell Culture → Exosome Isolation → Nucleic Acid/Protein Analysis”, catering to both the flexibility of scientific research and thedemands of standardized production.

High-Quality Cell Culture System – "Source Control" for Exosomes

Exosomes can be isolated directly from various body fluids for biomarker discovery or diagnostic studies. However, when the goal is to prepare exosomes from specific cell types (e.g., functional mechanism studies or therapeutic applications of mesenchymal stem cell-derived exosomes), the quality of the donor cell culture becomes the critical starting point determining the yield, purity and functional consistency of exosomes. Mesenchymal Stem Cells (MSCs) are currently the most commonly used therapeutic cell type in exosome research. The anti-inflammatory,

pro-angiogenic and immunomodulatory active components carried by MSC-derived exosomes are closely related to the cell state. When researchers aim to engineer MSC exosomes (e.g., loading drugs or targeting ligands) or perform GMP-grade production, a standardized, exogenous interference-free cell expansion system is essential. To this end, we have launched TransStem^® Serum-Free, Xeno-Free Human Mesenchymal Stromal Cell Medium (GMP Grade) specifically for human MSCs, providing a "pure, stable, high-yield" upstream guarantee for MSC-derived exosome production.

TransStem^® Serum-Free, Xeno-Free Human Mesenchymal Stromal Cell Medium (GMP Grade) (MM101 (with phenol red) / MM102 (without phenol red))

•High Expansion Efficiency: Small cell diameter, maintained between 15-17 µm; high cell yield, obtaining 10¹² - 10¹⁴ umbilical cord MSCs from P0 to P5.

•High Safety: Serum-free, xeno-free components.

•Good Efficacy: High differentiation potential into osteoblasts, chondrocytes and adipocytes; strong immunomodulatory capacity.

•High Versatility: Suitable for primary isolation and subculture expansion of MSCs from various sources such as umbilical cord, bone marrow and adipose tissue, as well as for subculture expansion of MSCs derived from human pluripotent stem cells.

•Pharmaceutical-Grade Raw Materials: Core raw material production complies with FDA cGMP standards and holds FDA DMF Type II filing.

Efficient Exosome Isolation Solutions

The isolation and purification of exosomes is a critical step determining the reliability of downstream experimental results. An ideal isolation method should satisfy: high recovery yield, high purity (low contamination from non- exosomal proteins/lipoproteins), preservation of exosome structural integrity and bioactivity, ease of operation and standardizability. Current mainstream exosome isolation methods each have their own advantages and disadvantages, briefly compared in the table below:

No single method fulfills all requirements simultaneously; the optimal choice depends on the sample type, downstream application purpose, and requirements for purity and recovery yield. We offer two complementary exosome isolation products tailored to different sample sources and experimental goals.

TransExo^® PS Exosome Isolation Kit (FE601)

•Simple operation, no ultracentrifugation needed; completes exosome purification within 3 hours.

•Higher specificity and purity compared to ultracentrifugation.

•Gentle elution conditions yield exosomes with good morphology, intact structure, and high activity.

Schematic Diagram

TransExo^® Cell Media Exosome Kit (FE401)

A product specifically for exosome extraction and purification from cell culture supernatant. The obtained exosomes exhibit high purity and high activity, suitable for various detection methods such as Western Blot, transmission electron microscopy, qPCR and particle size analysis.

•Simple operation, no ultracentrifugation needed.

•Low sample volume requirement, high sensitivity.

Precise Small RNA Analysis Tools

After obtaining high-purity, intact exosomes, molecular analysis of their contents is central to revealing functional mechanisms, discovering biomarkers or assessing therapeutic potential. Analysis of exosome cargo focuses primarily on two levels: small RNAs (especially miRNA, tRF, piRNA) and proteins. Due to the extremely low RNA content within exosomes (typically only 0.01-0.1 fg RNA per exosome) and the significant interference from residual ribonucleoproteins and genomic DNA in samples, specially optimized extraction and analysis methods are required.

EasyPure^® miRNA Kit (ER601)

•Improved operational safety: Uses RNA Extraction Agent instead of chloroform.

•Strong lysis capability, high extraction yield and broad application range.

•Suitable for miRNA and total RNA extraction from cells, tissues, fresh blood and exosomes.

•By adjusting the volume of absolute ethanol added to the aqueous phase, the following can be obtained:

1. RNA spin column adsorbs large molecular weight RNA (28S rRNA, 18S rRNA, mRNA), and then the flow-through (containing RNAs smaller than 200 nt, such as miRNA, siRNA, shRNA, snRNA, etc.) is applied to a miRNA spin column to adsorb Small RNA.

2. RNA spin column adsorbs all RNA (including RNA smaller than 200 nt).

TransScript^® Green miRNA Two-Step qRT-PCR SuperMix (AQ202)

•Optimized ratio of poly(A) polymerase and reverse transcriptase ensures miRNA reverse transcription efficiency.

•Poly(A) tailing and cDNA synthesis are completed in one step within the same reaction system.

•Uses PerfectStart^® Green qPCR SuperMix for amplification, offering strong amplification efficiency, high specificity,good sensitivity and accurate data.

Western Blot Related Products

Western Blot is a core experimental method for validating exosome purity and identity, typically requiring detection of exosomal marker proteins such as CD9, CD63, CD81 and TSG101. We have a complete general Western Blot product line, including products for protein quantification, electrophoresis & transfer, detection & imaging, supporting the entire workflow of exosome protein analysis.

Making Exosome Research Simpler and More Efficient

Whether you are an academic laboratory focusing on fundamental questions in exosome biology or an R&D  team dedicated to developing exosome-based diagnostics or therapeutics, our products can help you overcome technical bottlenecks and accelerate the process from "idea" to "data" to "application." In the future, we will continue to develop new products to further empower the vast prospects of exosome technology in precision medicine and regenerative medicine.

Related Product Information