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Porcine Reproductive and Respiratory Syndrome Virus North America Classical Type and Mutant Type PCR Nucleic Acid Detection Kit


Product Details

Using Taqman probe technology, specific primers and probes were designed based on the specific conservative genes of Porcine Reproductive and Respiratory Syndrome Virus North America Classical Type (PRRSV-C) and Porcine Reproductive and Respiratory Syndrome Virus Mutant Type (PRRSV-M) as the target sequences for the auxiliary detection of PRRSV-C and PRRSV-M nucleic acid in samples. This product introduces a dUTP/UDG mix that degrades U-containing ssDNA and dsDNA, eliminating cross-contamination caused by PCR products. At the same time, a positive internal control (an internal standard, using a VIC reporter group) is set to monitor whether the qPCR reaction contains PCR inhibitors by detecting whether the internal standard is normal to avoid false-negative results.


Notes: The Porcine Reproductive and Respiratory Syndrome Virus highly pathogenic variants in this kit has a feature that non-structural protein NSP2 encoding area is missing 30 amino acids. NANC 30 variant, its NSP2 protein 324-434, 486, 505-523 sites, have 111, 1, and 19 missing amino acids, which are "111+1+19" amino acid missing mode. 

There is no NSP2 gene missing in North American classic strains, and VR2332 variants used as standard variants.


Storage

Store in the dark at -20±5°C. Valid for 12 months.


Shipping

Dry ice (-20°C)

Product Contents


NameComponent

Specification

2× One-Step Reaction MixqPCR reaction buffers, dNTPs, PCR enhancers, etc.600 μl/tube

One-Step Enzyme Mix

UDG enzymes, reverse transcriptase, Taq enzymes

48 μl/tube
PRRSV-C/M Primer Probe Mix-2Primers, probes312 μl/tube
PRRSV-C/M Negative Control-2Nuclease-free Water400 μl/tube
PRRSV-C/M Internal Positive Control-2Nucleic acid containing amplified fragment of internal positive standard480 μl/tube
PRRSV-C/M Positvie Control-2Nucleic acid containing target amplified fragment50 μl/tube


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