PerfectStart^® V Fast Direct Probe qPCR SuperMix UDG is supplied at a 2× concentration, and is a pre-mix reagent designed specifically for probe-based qPCR detection of target genes without the need for nucleic acid extraction and purification steps. Simply add primers, probe and lysed samples to perform singleplex, duplex and multiplex PCR reactions in one reaction well. This product contains newly upgraded PerfectStart^® V Taq Hot-Start DNA Polymerase (Genetically modified Taq DNA polymerase with exceptionally high inhibitor tolerance), combined with optimal reaction buffer, which significantly enhances template affinity and qPCR amplification performance, improving tolerance to crude samples containing impurities and increasing  detection sensitivity. It is particularly effective for direct DNA amplification from samples that have not undergone nucleic acid extraction and purification, such as anticoagulated whole blood, plasma, serum, throat swabs. This product can support fast amplification, enabling PCR amplification to be completed in 35 minutes. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and ensure the accuracy of results.

• Hot start Taq DNA polymerase blocking by antibodies, enabling high specificity, high sensitivity, high amplification efficiency, and a wide range of applicable species.

• High-efficiency antibody blocking supports direct amplification from unextracted samples containing high concentrations of whole blood (5%-10%).

• Supports multiplex fast amplification, completing reactions in approximately 30 minutes.

• The dUTP/UDG anti-contamination system is used to effectively prevent carry-over contamination of PCR products, ensuring the accuracy of results.

• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors.

Suitable for probe-based qPCR detection of lysed samples from various sources, including animal, plant, and microbial samples.

at -18℃ or below for two years

Dry ice (-70℃)