Y-27632 is a highly potent, cell-permeable, reversible and selective inhibitor of p160ROCK (Rho-associated, coiled-coil
domain-containing protein kinase, ROCK). Y-27632 inhibits the kinase activity of both ROCK1 and ROCK2 with almost equal
potency. The inhibition is achieved by competing with ATP for binding to the catalytic site. Y-27632 prevents cell apoptosis,
enhances survival and cloning efficiency of human pluripotent stem cells when they are dissociated to single cells, and improves
the efficiency of embryoid body formation using the forced aggregation method.
Storage
at -20℃ in the dark for six months, avoid repeated freeze-thawing.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
