RNA Purification
  • ER201-01.JPG
ER201-01.JPG

EasyPure® Viral DNA/ RNA  Kit


Product Details

EasyPure® Viral DNA/RNA Kit provides a simple and fast column based method to isolate viral DNA/RNA from up to 200 μl of plasma, serum, body fluid and mammalian cell supernatant. Samples are lysed with unique lysis buffer and DNA/RNA is enriched by carrier RNA. DNA/RNA is bound to silica membrane. After washing, high quality DNA/RNA is eluted from the column. DNA/RNA is free of protein contamination, and is suitable for PCR, RT-PCR, qPCR and qRT-PCR.

Storage
Carrier RNA at -20°C for one year; Proteinase K solution at -20°C for two years; others at room temperature (15°C-25°C ) for one year.

Shipping
Proteinase K  and Carrier RNA on dry ice (-70 °C); others at room temperature.

Product Contents

ComponentER201-01 (50 rxns)
Binding Buffer 5 (BB5)15 ml
Wash Buffer 5 (WB5)12 ml
Proteinase K (20 mg/ml)1 ml
Carrier RNA (1 μg/μl)310 μl
RNase-free Water10 ml
RNase-free Tube (1.5 ml)50 each
RNA Spin Columns with Collection Tubes50 each

References

Bingqing L , Yingying Y , Yajie Z , et al. A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity[J]. Frontiers in Cellular and Infection Microbiology, 2017, 7:26.
Wu Y , Cui X , Wu N , et al. A unique case of human Zika virus infection in association with severe liver injury and coagulation disorders[J]. Scientific Reports, 2017, 7(1):11393.
Dongfang H , Lin L , Jinyuan G , et al. Genetic Diversity and Positive Selection Analysis of Classical Swine Fever Virus Envelope Protein Gene E2 in East China under C-Strain Vaccination[J]. Frontiers in Microbiology, 2016, 7:85.
Gao Y , Li Y , Meng Q , et al. Serum HBV DNA, RNA and HBsAg: which correlated better to intrahepatic covalently closed circular DNA before and after nucleos(t)ide analogue treatment[J]. Journal of Clinical Microbiology, 2017:JCM.00760-17.
Chen S , Luo G , Yang Z , et al. Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways[J]. Veterinary Research, 2017, 47(1):74.

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