RNA Purification
  • ER301-01.JPG
ER301-01.JPG

EasyPure® Plant RNA Kit


Product Details

EasyPure® Plant RNA Kit provides a simple and fast column based method to isolate RNA from plant tissue. Samples are lysed with detergent to inactivate RNase. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column. RNA is free of protein contamination, and is suitable for RTPCR, qRT-PCR, Microarray analysis and Northern blot.


Storage
DNase I and DNase I Reaction Buffer at -20°C for one year; others at room temperature (15-25°C) for one year.

Shipping
DNase I and DNase I Reaction Buffer on dry ice (-70 °C); others at room temperature.

Product Contents

ComponentER301-01 (50 rxns)
Binding Buffer 6 (BB6)60 ml
Wash Buffer 6 (WB6)12 ml
Clean Buffer 6 (CB6)60 ml
DNase I (3 units/μl)1500 units
DNase I Reaction Buffer4×1 ml
RNase-free Water10 ml
RNase-free Tube (1.5ml)50 each
RNA Spin Columns with Collection Tubes50 each

References

Gulati J, Kim SG, Baldwin IT, et al. Glutamate Receptor Like Channel 3.3 is involved in mediating glutathione-triggered cytosolic Ca3+ transients, transcriptional changes and innate immunity responses in Arabidopsis[J]. Plant Physiology, 2013, 162(2):1042-59.
Liu Y , Yang S , Song Y , et al. Gain-of-function analysis of poplar, CLE, genes in Arabidopsis by exogenous application and over-expression assays[J]. Journal of Experimental Botany, 2016, 67(8):2309-24.
Chen S , Yuan N , Schnabel G , et al. Function of the genetic element 'Mona' associated with fungicide resistance in Monilinia fructicola[J]. Molecular Plant Pathology,  2017, 18(1):90-97.
Vijayata S , Artemis P , Kim S Y , et al. Tyrosine-610 in the Receptor Kinase BAK1 Does Not Play a Major Role in Brassinosteroid Signaling or Innate Immunity[J]. Frontiers in Plant Science, 2017, 8:1273.
Tongjin L , Xiaohui Z , Haohui Y , et al. Aromatic Glucosinolate Biosynthesis Pathway in Barbarea vulgaris and its Response to Plutella xylostella Infestation[J]. Frontiers in Plant Science,  2016, 8(7):83.

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