RNA Purification
  • ER601-01.JPG
ER601-01.JPG

EasyPure® miRNA Kit


Product Details

EasyPure® miRNA Kit provides a simple and fast column based method to isolate small RNA (≤200 nt) from cells, tissues, fresh blood and virus. Samples are lysed with lysis buffer. The addition of chloroform to the sample separates the solution into an upper colorless aqueous phase containing RNA, an interphase and a lower organic phase. High molecular RNA (28S rRNA, 18S rRNA, mRNA) is bound to a silica membrane. Small RNA in the flow-through can be bound to a miRNA spin column.

Storage
LB10 at 2-8°C in dark for one year; others at room temperature (15°C-25°C) for one year.

Shipping
LB10 on ice bag (4 °C); others at room temperature.

Product Contents

ComponentER601-01 (50 rxns)
Lysis Buffer10 (LB10)55 ml
Wash Buffer 10 (WB10)12 ml
RNA Spin Columns with Collection Tubes50 each
miRNA Spin Columns with Collection Tubes50 each
R N a s e - f r e e T u b e ( 1.5 ml)50 each
RNase-free Water10 ml

References

Wu H , Li Z , Yang P , et al. PKM2 depletion induces the compensation of glutaminolysis through β-catenin/c-Myc pathway in tumor cells[J]. Cellular Signalling, 2014, 26(11):2397-2405.
Zhang J , Xu Y , Cao T , et al. Arsenic methylation by a genetically engineered Rhizobium-legume symbiont[J]. Plant and Soil, 2017, 416(1-2):259-269.
Yan Y , Song X , Li Z , et al. Elevated levels of granzyme B correlated with miR-874-3p downregulation in patients with acute myocardial infarction[J]. Biomarkers in Medicine, 2017, 11(9):761-767.
Wang J , Yan Y , Song D , et al. The association of plasma miR-155 and VCAM-1 levels with coronary collateral circulation[J]. Biomarkers in Medicine, 2017, 11(2):125-131.
Zhang Y , Weng S , Yin J , et al. Vitamin K2 promotes mesenchymal stem cell differentiation by inhibiting miR?133a expression[J]. Molecular Medicine Reports, 2017, 15(5):2473-2480.

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