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Trans reverse transcriptases (Trans RT) contains three categories of reverse transcriptases. They are all in absence of RNaseH activity to reduce RNA template degradation during the first-strand cDNA synthesis. Each Trans RT has its corresponding first-strand cDNA synthesis SuperMix, Two-Step and One-Step RT-PCR SuperMix. One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination.